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This is Scientific American 60-Second Science. I'm Christopher Intagliata. Got a minute?
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In this age of cheap DNA technology, scientists are sequencing every sample they can get their hands on.
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They've ID'd the microbes in mosquito guts, coral mucus and frog skin; in polar ice; even floating in the Earth's atmosphere.
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But it turns out some of the bugs reported to belong to those unusual microbiomes could unfortunately be contaminants, from non-sterile lab reagents and DNA extraction kits.
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So says a study in the journal BMC Biology.
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Researchers sequenced a pure sample of just one type of bacteria.
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But depending which kit they used, which reagents, which lab, their results contained DNA from up to 270 different bacterial strains.
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Many of those contaminating strains are commonly found on human skin¡ (a lab technician's, maybe?).
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Or in soil or water.
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Which could explain why one recent study turned up soil bacteria in samples of breast cancer tissue, the researchers say.
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Another study found that infants' throat bacteria change as they get older.
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But these researchers say the changing bacterial communities in that study were due not to age, but to changing the brand of DNA kit over time.
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Study author Alan Walker, of the University of Aberdeen, says contamination is only a problem if you're working with samples that aren't already rich in bacteria.
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"If you're doing fecal work, for example, this probably doesn't concern you,
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because there's enough DNA coming from the actual sample that it'll drown out any of the background contamination."
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His recommendation for scientists?
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Alongside the actual samples, try sequencing nothing¡ to see what sort of shadow microbiome is already lurking in your lab.
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Thanks for the minute, for Scientific American 60-Second Science. I'm Christopher Intagliata.
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1 / 18
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显示原文 =This is Scientific American 60-Second Science. I'm Christopher Intagliata. Got a minute?
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2 / 18
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显示原文 =In this age of cheap DNA technology, scientists are sequencing every sample they can get their hands on.
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3 / 18
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显示原文 =They've ID'd the microbes in mosquito guts, coral mucus and frog skin; in polar ice; even floating in the Earth's atmosphere.
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4 / 18
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显示原文 =But it turns out some of the bugs reported to belong to those unusual microbiomes could unfortunately be contaminants, from non-sterile lab reagents and DNA extraction kits.
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5 / 18
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显示原文 =So says a study in the journal BMC Biology.
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6 / 18
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显示原文 =Researchers sequenced a pure sample of just one type of bacteria.
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7 / 18
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显示原文 =But depending which kit they used, which reagents, which lab, their results contained DNA from up to 270 different bacterial strains.
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8 / 18
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显示原文 =Many of those contaminating strains are commonly found on human skin¡ (a lab technician's, maybe?).
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9 / 18
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显示原文 =Or in soil or water.
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10 / 18
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显示原文 =Which could explain why one recent study turned up soil bacteria in samples of breast cancer tissue, the researchers say.
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11 / 18
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显示原文 =Another study found that infants' throat bacteria change as they get older.
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12 / 18
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显示原文 =But these researchers say the changing bacterial communities in that study were due not to age, but to changing the brand of DNA kit over time.
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13 / 18
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显示原文 =Study author Alan Walker, of the University of Aberdeen, says contamination is only a problem if you're working with samples that aren't already rich in bacteria.
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14 / 18
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显示原文 ="If you're doing fecal work, for example, this probably doesn't concern you,
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15 / 18
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显示原文 =because there's enough DNA coming from the actual sample that it'll drown out any of the background contamination."
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16 / 18
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显示原文 =His recommendation for scientists?
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17 / 18
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显示原文 =Alongside the actual samples, try sequencing nothing¡ to see what sort of shadow microbiome is already lurking in your lab.
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18 / 18
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显示原文 =Thanks for the minute, for Scientific American 60-Second Science. I'm Christopher Intagliata.
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- 单句循环：关
- 单句循环：×3
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- 单句循环：∞